method of soil sterilization

Soil sterilization

"Sterilization" is the removal or killing of all the life forms from a substrates or medium. The term "Sterilization" is absolute in itself, meaning, a medium may either be "sterilized" or "unsterilized". In other words, "sterilization" can never be semi, partial or half.

Soil sterilization

Soil is a very common substrate for the plants since the evolution of first land plant during palaeozoic era. A soil can harbor plenty of soil microbes, which eventually constitute below ground biodiversity. The soil microbes (including bacteria, fungi, nematodes) may be beneficial, natural or harmful.
Harmful microbes cause substantial loss in the yield and economy. The soft targets of harmful microbes are always seedlings and young growing plantlets. It becomes paramount important to make the nursery substrate free from harmful microbes. This can be achieved through the sterilization of nursery soil. Different soils have different compostions, textures, densities, nutrients and living organisms. Soils are very hard to penetrate by the sterilizing agents. Sterilization also kills the beneficial microbes too. There are other techniques (e.g., tyndallization) which kill only the harmful microbes, but not the useful ones. These techniques can be adapted to make soils and nursery beds healthy and free from harmful microbes. Soil sterilization is a very challenging job.
Sterilization is the misnomer of all these techniques and adapted in general used for making soils free of harmful microbes.
Below are the techniques used for making soil free of harmful microbes.


An autoclave is idealy a large-scale pressure cooker, that runs on the principle of "steam-underpressure". Very first autoclave was developed by Charles Chamberland, a pupil of Louis Pasteur. It is laboratory instrument and is usefull for the sterilization of small quantities of soil for the nursery beds and experimentation. Autoclave is a closed chamber for wet-heating. In autoclave various temperature can be achieved at different pressure.
Table showing relationship between the pressure and temperature of steam
Pressure (psi) Temperature (°C)

When maintained for 30 minutes, the steam in the autoclave penetrate the soil and killed various organisms at different temperature.
Table showing organisms killed at temperture sustained for 30 minutes
Temperature (°C) Organisms killed
48.8Water molds
62.7Most pathogenic bacteria, worms, centipeded
71.1All pathogenic bacteria
82.2Seeds of weeds
100Heat resistant weed seeda dn viruses
121Heat-resistant endospores of bacteria

Autoclaving is an easy and fast method of soil sterilization. Autoclaving does not change the organic content and pH of the soil. However, it has adverse effects on soil texture.

Method of soil autoclaving